ETF1 antibody

Catalog No. ABIN2720568

The Mouse Monoclonal anti-ETF1 antibody has been validated for WB and FACS. It is suitable to detect ETF1 in samples from Human.

Quick Overview for ETF1 antibody (ABIN2720568)

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ETF1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 2E3

Product Details anti-ETF1 Antibody

Characteristics: Homo sapiens eukaryotic translation termination factor 1 (ETF1)

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.

Isotype: IgG2a

Application Details

Application Notes: WB 1:500, FLOW 1:100,

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for ETF1

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Alternative Name: ETF1

Background: Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).

Molecular Weight: 48.9 kDa

Gene ID: 2107

NCBI Accession: NM_004730

HGNC: 2107